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Biology Group Research Article Article ID: igmin325

Purification of Monoacylglycerides using Urea

Plant Biology DOI10.61927/igmin325 Affiliation

Affiliation

    R&D Laboratory, Behshahr Industrial Company, 8th Km, Fath Highway, Tehran, Iran

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Abstract

Monoacylglycerides (MAGs) produced during conventional synthesis contain impurities such as diacylglycerides, triacylglycerides, and glycerol. In this study, urea was employed to form complexes with MAGs derived from palm oil (PO), canola oil (CO), fully hydrogenated palm oil (FHPO), and fully hydrogenated soybean oil (FHSO) in hot methanol. After crystallization and separation of the urea–MAG complexes, hydrochloric acid was used to release purified MAGs. The results demonstrated a significant increase in α-MAG content (from 39.8%, 40.4%, 42, 4 and 43.4% to 74%, 85.4%, 83.1%, and 51.9% for PO, FHPO, FHSO, and CO, respectively, p < 0.05). These results confirm the effectiveness of the urea complexation method for α‑MAG purification.

References

    1. O’Brien RD. Fats and oils: formulating and processing for applications. Boca Raton (FL): CRC Press. 2003;268–270.
    2. Bennion EB, Bamford GST. The technology of cakemaking. London (UK): Blackie Academic & Professional. 1997; 114–115.
    3. Jungermann E, Sonntag NOV. Glycerine: a key cosmetic ingredient. New York (NY): Marcel Dekker. 1990;313–314.
    4. Stauffer CE. Functional additives for bakery foods. Gaithersburg (MD): Aspen Publishers. 1990;88–89.
    5. Hui YH. Bailey’s industrial oil & fat products. Vol. 4, Edible oil & fat products: processing technology. New York (NY): Wiley. 1996;572–592.
    6. Alsop WG. Process for the preparation of higher fatty acid monoglycerides. US patent 3083216. 1963.
    7. Kuhrt NE, Welch EA, Kovarik FJ. Molecularly distilled monoglyceride I. Preparation and properties. J Am Oil Chem Soc. 1950;27:310–317.
    8. Lkan R. Natural products: a laboratory guide. Amsterdam (Netherlands): Elsevier. 1991;39–41.
    9. American Oil Chemists’ Society. Official methods and recommended practices of the AOCS. 5th ed. Champaign (IL): AOCS. 1998.

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